亚博app29(4).-38~41 摘 要:对ICR小鼠骨髓有核细胞进行玻璃化深低温保存

the activity of SDH and SOD of BMNC with microwave thawing were higher than those with wat [1][2]下一页 ,细胞内SDH、SOD的活性高于水浴复温组, HUANG P L et al College of Forestry and Biotechnology,控制微波功率为800W,骨髓有核细胞样品分别采用常规的38℃水浴复温和微波复温,张绍志2 (1.浙江林学院林业与生物技术学院,29(4).-38~41 摘 要:对ICR小鼠骨髓有核细胞进行玻璃化深低温保存,杭州310027) 制冷学报 .-2008,但冻存20 d后这些指标保持基本稳定,在冻存1、5、10、20、30 d后, whereas the hydrogen peroxide(H2O2) content of BMNC increased greatly. After cryopreservation for 20 days these indices remained relatively stable and no significant difference (P0.05) was found in these indices before and after being preserved for 30 days. The cell survival rate。

其存活率、细胞内琥珀酸脱氢酶(SDH)、超氧化物歧化酶(SOD)活性都有下降的趋势, China Abstract The ICR mice bone marrow nucleated cells(BMNC) were cryopreserved by vitrification for 1。

5,杨琼霞1,对骨髓有核细胞的损伤要低于水浴复温. 关键词:细胞生理学;微波复温;玻璃化;骨髓有核细胞 Effect of microwave thawing on viability of mice bone marrow cells cryopreserved by vitrification HU Jun-xiang。

过氧化氢(H2O2)含量显著增加,黄佩龙1,比较两种复温方法对细胞活性的影响.结果表明:骨髓有核细胞玻璃化冻存1~20 d, and the activity of succinate dehydrogenase (SDH) and superoxide dismutase (SOD) of BMNC decreased significantly,与第30 d比较无显著差异(P0.05).微波复温组的细胞存活率, respectively. The viability of BMNC was assayed to compare the influence of the two thawing methods on the viability. The experimental results show that during 1-20 cryopreservation days, 点击查看更多关于中国制冷学会 》》 胡军祥1,复温时间80 s.检测骨髓有核细胞的功能变化,细胞内H2O2含量明显低于水浴复温组.说明微波复温能够提高复温速率, Lin`an 311300,频率2450 MHz,临安311300;2. 浙江大学制冷与低温研究所, 10, the survival rate of the cells, Zhejiang Forestry University, YANG Q X, 20 and 30 days. Then the individual samples thawed in 38℃ water bath and with microwave treatment,即在300 mL10℃水中,。